بررسی آنتی‌بادی‌های تولید شده علیه آنتی‌ژن‌های کیست هیداتیک با استفاده از روش ایمونوبلات
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Abstract:
Introduction: Immunoblot is recognized as a sensitive and specific serological method for diagnosis of human hydatidosis. To analyze the reactivity of hydatid patients sera with the subunits of 2 major hydatid cyst fluid antigens, ( 5 and B ) immunoblot method was used. Material & Methods:In this regard, 100 sera ,of which 40 sera from patients with surgically diagnosed hydatidosis, 20 sera from patients with parasitic disease other than hydatidosis, 20 sera from patients other than parasitic disease, and 20 sera from healthy controls were examined. Results: For 3 subunits of Ag B (12, 16, 20 kD) the sensitivities were 92.5%, 84.5% and 87.5% respectively and their specificity for all of the 3 subunits was 100%. For 2 subunits of Ag 5, (55, 65 kD) the sensitivity was 100% and the specificities were 100% and 90% respectively . Conclusion: The results indicated that the best subunit for hydatidosis diagnosis is the 55 kD subunit of Ag 5. Meanwhile, a lower reactivity (84.5% of all hydatid sera) was observed with 16 kD subunits of Ag B.
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بررسی آنتی بادی های تولید شده علیه آنتی ژن های کیست هیداتیک با استفاده از روش ایمونوبلات
مقدمه : ایمونوبلات یکی از روشهای سرولوژی با حساسیت و ویژگی بالا است که برای تشخیص بیماری کیست هیداتیک بکار می رود. در این روش سرم بیماران هیداتیدی با زیر واحدهای دو آنتی ژن اصلی اکینوکوکوس گرانولوزوس یعنی آنتی ژن ب وآنتی ژن 5 واکنش ایجاد می کند و پاسخهای ایجاد شده قابل تجزیه و تحلیل می باشند. روش بررسی : در این بررسی تعداد 100 سرم مورد آزمایش قرار گرفتند که 40 مورد مربوط به بیماران هیداتیدی می ...
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Background: Diabetes mellitus increases the risk of central nervous system (CNS) disorders such as stroke, seizures, dementia, and cognitive impairment. Berberine, a natural isoquinolne alkaloid, is reported to exhibit beneficial effect in various neurodegenerative and neuropsychiatric disorders. Moreover astrocytes are proving critical for normal CNS function, and alterations in their activity...
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Introduction & Objective : Hydatidosis, a disease caused by the cestod helminth echinococcus granulosus, is one of the most important parasitic zoonosis in man and a variety of animals. Sensitive and reliable serologic methods are necessary to confirm the diagnosis. In this study, Ag B and Psc Ag were purified as two specific parasitic antigens and evaluated by Dot blotting used on the serum of...
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Journal title
volume 12 issue 2
pages 33- 38
publication date 2004-07
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